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Puncta analysis of the R110A mutant. (A) P-value calculation for quantitative R110A puncta-formation analysis (Additional file 3: Table S1, Additional file 4: Figure S3, Additional file 5: Figure S4) with regard to CM: ** < 0.01; with regard to wild-type GFP-WIPI-1: ### < 0.001. (B) 50 puncta structures of GFP-WIPI-1 or GFP-R110A were categorized (1–4). Further supplementary material is available (Additional file 7: Figure S5).
