Table 1. Summary of in vitro preparation of cell-scaffold constructs.

Group	Seeding method	Culture condition	Cell suspension concentration per scaffold	Cell suspension volume per scaffold	Cell number per scaffold	Seeding efficiency (%)
A	Hydrodynamic	Hydrodynamic	1.0×106 cells/ml	1.00 ml	1.0×106	32.10±0.72#
B	Hydrogel-assisted	Hydrodynamic	2.0×107 cells/ml	0.05 ml	1.0×106	82.14±1.09
C	Static	Static	2.0×107 cells/ml	0.05 ml	1.0×106	25.24±1.56*
D	Hydrogel-assisted	Static	2.0×107 cells/ml	0.05 ml	1.0×106	81.53±1.37

^*: seeding efficiency in group C was lower than that in other groups (p<0.05);

^#: seeding efficiency in group A was lower than that in group B and D (p<0.05).