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| Group | Scaffold | Seeding condision | Cell number per scaffold | Culture time | Place in nude mouse |
| I | DBM | no | no | no | left rostral |
| II | DBM | Hydrogel-assisted | 1.0×106 | dynamic culture 12 d | right rostral |
| III | DBM | Hydrogel-assisted | 1.0×107 | no | left caudal |
| IV | DBM | Hydrogel-assisted | 1.0×106 | static flask culture 12 d | right caudal |
