Figure 1. Tumor growth and survivability of NLGP-treated mice.

A.1. Swiss mice were inoculated with Sarcoma 180 cells (1×10⁶ cells/mice). After formation of palpable tumor, mice of experimental group (n = 24) were treated with NLGP (25 µg) once a week for 4 weeks in total and other control group (n = 24) received PBS only. Pattern of tumor growth (in mm³) of each mouse is presented till day 28 after the initiation of therapy. Arrows indicate the points of NLGP injection A.2. Mice were inoculated with s.c. sarcoma and after formation of tumors mice of experimental group were treated with NLGP (25 µg) once a week for 4 weeks in total. Mean tumor volume of PBS- and NLGP-treated mice is presented (n = 24, in each case) (*p<0.05; **p<0.001), along with their mean survival till day 90 (⁺ p<0.001). B.1. Mice were inoculated with Sarcoma 180, after formation of palpable tumors, mice were randomly divided into five groups (n = 6, in each group) and treated with different doses of NLGP (12 µg, 25 µg, 50 µg and 100 µg) once a week for 4 weeks in total and PBS for fifth group. Tumor growth curve (*p<0.01 for 25 µg; ^♦ p<0.01 for 50 µg) and their mean survival are presented (⁺ p<0.01). B.2. Mice were inoculated with Sarcoma 180; after the formation of the palpable tumors, mice were randomly grouped into four (n = 6, in each group) and treated with NLGP (25 µg) s.c. for various time intervals (3 days, 7 days and 15 days) and 4 injections were given in each treatment cohort. Fourth group was kept untreated. Tumor growth curve (*p<0.001) and their mean survival (⁺ p<0.01) are presented. B.3. Mice were inoculated with Sarcoma 180; after the formation of the palpable tumors, mice were randomly grouped into five (n = 6, in each group) and treated with NLGP, through different routes (subcutaneous, intraperitoneal, intravenous and intratumoral) once a week for 4 weeks in total. Tumor growth curve (*p<0.01, s.c. and i.p.; ^♦ p<0.01, iv) and their mean survival (⁺ p<0.01) are presented. B.4. Mice were inoculated with Sarcoma 180; after the formation of the palpable tumors, mice were randomly grouped into three (n = 6, in each group) and treated with NLGP (25 µg) (single vs four weekly injections). Tumor growth curve (*p<0.001) and their mean survival (⁺ p<0.01) are presented.