Table 2. Primer sequences of various genes studied.
Name | Primer sequence (5′-3′) | Product size |
CXCR3-forward | GCTGCTGTCCAGTGGGTTTT | 67 bp. |
CXCR3-reverse | AGTTGATGTTGAACAAGGCGC | |
CXCL9-forward | TGGGCATCATCTTCCTGGAG | 204 bp. |
CXCL9-reverse | CCGGATCTAGGCAGGTTTGA | |
CXCL10-forward | CCAAGTGCTGCCGTCATTTT | 177 bp. |
CXCL10-reverse | CTCAACACGTGGGCAGGATA | |
CCR5-forward | ACTGCTGCCTAAACCCTGTCA | 78 bp. |
CCR5-reverse | GTTTTCGGAAGAACACTGAGAGATAA | |
CCL3-forward | CCTCTGTCACCTGCTCAACA | 163 bp. |
CCL3-reverse | GATGAATTGGCGTGGAATCT | |
CCL4-forward | GCTGTGGTATTCCTGACCAAA | 196 bp. |
CCL4-reverse | AAATCTGAACGTGAGGAGCAA | |
CCL5-forward | CCCTCACCATCATCCTCACT | 186 bp. |
CCL5-reverse | TCCTTCGAGTGACAAACACG | |
CCL8-forward | ACGCTAGCCTTCACTCCAA | 231 bp. |
CCL8-reverse | TCTGGAAAACCACAGCTTCC | |
Perforin-forward | GATGTGAACCCTAGGCCAGA | 161 bp. |
Perforin-reverse | GGTTTTTGTACCAGGCGAAA | |
Granzyme B-forward | TCGACCCTACATGGCCTTAC | 198 bp. |
Granzyme B-reverse | TGGGGAATGCATTTTACCAT | |
TIM3-forward | CCCCTGCCAAGTACTCATGT | 161 bp. |
TIM3-reverse | CAAGTGCCCCAGGTGTAGAT | |
PD1-forward | CATGCCCAGGTACCTCAGTT | 236 bp. |
PD1-reverse | GAACCCAACTCCAGGACAGA | |
LAG3-forward | TTCCGGCTCAGAGGAAGATA | 200 bp. |
LAG3-reverse | TTTTTGATGCTGCTGACAGG | |
β-Actin-forward | CAACCGTGAAAAGATGACCC | 228 bp. |
β-Actin-reverse | ATGAGGTAGTCTGTCAGGTC |