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. 2013 Jan 11;8(1):e52345. doi: 10.1371/journal.pone.0052345

Figure 2. ChemLTP upregulates of SUMO-1 and Ubc9 protein levels.

Figure 2

A) ChemLTP increases in SUMO-1 and Ubc9 immunoreactivity with no change in the intensity of SUMO-2 staining. Mean fluorescence intensity was analysed using ImageJ. Graphs show quantification of 3 independent experiments, 10 neurons per condition (ANOVA. *p<0.02; **p<0.005). Scale bars – 20 µm. B) ChemLTP enhances colocalization between SUMO-1 and Ubc9, and SUMO-2/3 and Ubc9 in cultured hippocampal neurons. Example of dendritic segments stained for SUMO-1 (upper panel) or SUMO-2 (lower panel) protein (blue) and Ubc9 (red). Colocalized puncta were quantified using JACoP plugin in ImageJ. Analysis was made in at least 3 different ROIs for each neuron (n = 10), for each condition in 3 independent experiments. (ANOVA. *p<0.02; **p<0.005). C) ChemLTP increases SUMO-1 clusters at synapses. Examples of fluorescence images of dendrites stained for SUMO-1 and PSD95. The colocalized clusters are indicated as yellow puncta. Graph shows quantification of SUMO-1/PSD95 colocalizing pixels. Analysis was performed in at least 3 independent experiments with 10 neurons per condition and 3 ROIs per neuron, using JACoP plugin in ImageJ. Statistical significance was determined using ANOVA. *p<0.02; **p<0.005.