Table 1. Evaluation of CXCR4 expression with flow cytometric analysis and IHC.

	MFIR CXCR4+ and CXCR4++ population	Ratio MFIR MIN-O/4T1	Ratio membranous staining MIN-O/4T1 (ex vivo)
MIN-O (early stage)	165.5±13.8	1.8	1.1
MIN-O (intermediate stage)	329.6±33.6	3.6	3.5
MIN-O (late stage)	367.9±22.4	4.0	5.5
4T1	91.5±14.4	-	-

For MIN-O tumor tissue, 6 biopsy samples per stage were evaluated. Also, n = 6 4T1 biopsy samples were assessed. All samples were evaluated in triplicate. MFIR: mean fluorescent intensity ratio. Ratio of membranous staining calculated from data reported in [15].