Skip to main content
. Author manuscript; available in PMC: 2014 Jan 7.
Published in final edited form as: Curr Biol. 2012 Nov 21;23(1):11–20. doi: 10.1016/j.cub.2012.10.047

Figure 6. TAAR5 and trimethylamine are required for mouse scent attraction.

Figure 6

(A) Expression of Taar5 and lacZ in olfactory sensory neurons of Taar5−/−, Taar5+/−, and Taar5+/+ mice visualized by two-color in situ hybridization. Scale bar = 50 μm. (B) Responses of Taar5+/+ and Taar5−/− mice to trimethylamine were measured using the two-choice odor compartment assay (mean ± s.e.). Experiments involved equal numbers of males and females, except as noted in Experimental Procedures. (C) Male urine was depleted of trimethylamine by incubation with FMO3-containing microsomes. Effective depletion was shown by an inability of TMA-depleted male urine to activate TAAR5 in HEK-293 cells at dilutions indicated (triplicates ± s.e.). (D) Behavioral responses of mice to stimuli indicated (40 μl) using the two-choice compartment assay (mean ± s.e.). (E) Behavioral responses of female mice (n=8-10, ± s.e.) of genotypes indicated to mock-depleted male urine (40 μl). (*p < 0.05, **p < 0.01)