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. Author manuscript; available in PMC: 2014 Jan 16.
Published in final edited form as: FEBS Lett. 2012 Nov 21;587(2):142–149. doi: 10.1016/j.febslet.2012.11.005

Figure 1. Mutation in helix 7j causes nuclear localization of clathrin hub.

Figure 1

A) Immunofluorescence staining of HA-hub WT and HA-hub C1573A in HEK293T, H1299, HeLa, and MCF7 cells indicates HA signal (green) is localized to membrane/cytoplasm in the HA-hub WT (leftmost panels) and C1573A (right panels). HA signal is also present in the nuclei (counterstained with Draq5 in blue) of nearly all cells expressing HA-hub C1573A (right panels) but not WT cells. B) Number of cells with nuclear HA signal is much higher (over 90% of cells) in cells expressing HA-hub C1573A compared to HA-hub WT cells. Error bars are SEM. ***p<0.001. C) Immunofluorescence in H1299 of HA-clathrin 865-1675 WT (left) and C1573A (right). HA signal is present in the nuclear spaces in HA-clathrin 865-1675 C1573A but not WT cells. Scale bar is 10 μm.