Table 1.
Analysis of WT and C1573A hub by multi-angle light scattering
| MM P1a (kDa) | Fb (%) | Rc (nm) | MM P2a | F (%) | R (nm) | MM P3a | F (%) | R (nm) | MM P4a | F (%) | R (nm) | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
| ||||||||||||
| C1573A | ||||||||||||
| Exp 1 | 551.1 | 25.3 | 23.6 | 276.0 | 21.4 | 16.4 | 167.4 | 42.9 | 13.0 | - | - | - |
| Exp 2 | 529.9 | 28.9 | 22.8 | 270.5 | 20.8 | 15.5 | 161.5 | 39.5 | 11.8 | - | - | - |
| Exp 3d | 492.9 | 3.3 | 27.8 | 232.8 | 6.7 | 18.6 | 147.6 | 10.6 | 10.6 | 74.2 | 44.3 | 8.4 |
| Mean SD | 524.63 ±29.46 | n/d | 24.7 ±2.7 | 259.77 ±23.52 | n/d | 16.8 ±1.6 | 158.83 ±10.17 | n/d | 11.8 ±1.2 | - | - | - |
|
| ||||||||||||
| C1573A + LCb | ||||||||||||
| Exp 1 | 515.3 | 25.8 | 25.7 | 209.5 | 31.6 | 16.4 | 129.6 | 13.7 | 16.9 | - | - | - |
| Exp 2 | 468.3 | 27.9 | 22.9 | 207.0 | 33.6 | 15.3 | 128.0 | 12.6 | 16.1 | - | - | - |
| Exp 3d | 536.9 | 5.5 | 28.5 | 199.9 | 27.9 | 15.0 | 110.6 | 52.9 | 14.2 | - | - | - |
| Mean SD | 506.83 ±35.08 | n/d | 25.7 ±2.8 | 205.47 ±4.98 | n/d | 15.6 ±0.74 | 122.73 ±10.54 | n/d | 15.7 ±1.4 | - | - | - |
| MM P1a (kDa) | Fb (%) | Rc (nm) | MM P2a | F (%) | R (nm) | |
|---|---|---|---|---|---|---|
|
| ||||||
| WT hub | ||||||
| Exp 1 | 210.2 | 100 | 13.2 | - | - | - |
| Exp 2e | 221.2 | 76.9 | 11.4 | 80.5 | 23.1 | 8.2 |
| Exp 3e,f | 237.0 | 65.4 | 11.8 | 111.2 | 20.1 | 11.0 |
| Mean SD | 222.8 ± 13.47 | n/d | 12.1 ± 1.0 | n/d | n/d | n/d |
|
| ||||||
| WT hub + LCb | ||||||
| Exp 1 | 262.0 | 75.9 | 12.1 | 164.9 | 9.9 | 10.3 |
| Exp 2e | 240.5 | 68.9 | 11.3 | 93.7 | 31.1 | 7.7 |
| Exp 3e | 261.2 | 60.1 | 10.7 | 102.4 | 23.9 | 9.2 |
| Mean SD | 254.57 ± 12.19 | n/d | 11.3 ± 0.7 | n/d | n/d | n/d |
a
Molar mass moments of peaks in Fig. 2.
b
Means of mass fractions not calculated to show sample dependent variability.
c
Average rms radius moments (radius of gyration) in nanometers.
d
Stored several days before measurements.
e
Sample in 10 mM Tris, 60 mM NaCl, 1 mM EDTA, pH 7.9 buffer.
f
Minor shoulder not tabulated.