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N2O production in vitro from (A) intact chloroplasts (nmol N2O-N g−1 chlorophyll s−1), (B) nitrate reductase (nmol N2O-N g−1 protein s−1), and (C) nitrite reductase (nmol N2O-N g−1 protein s−1). Intact chloroplasts, nitrate reductase, and nitrite reductase were extracted from fully expanded leaves of 2- to 3-week-old wheat plants (T. aestivum L. cv. Veery 10).
