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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Immunol Res. 2013 Mar;55(1-3):217–230. doi: 10.1007/s12026-012-8364-9

Fig. 1. DBA/1LacJ mice treated with CFA, but not IFA or alum, show preferential expansion of the canonical Vγ4Vδ4+ γδ T cell subset.

Fig. 1

DBA/1LacJ were mice immunized intradermally with collagen/CFA (denoted CIA), or with CFA, IFA, or alum only emulsified in PBS, and the draining lymph nodes harvested on the day indicated. Untreated controls (naïve) are shown for comparison A. Vγ4 transcripts from the lymph nodes of four CFA-treated males, immunized twice on d. 0 and 21 and sacrificed on day 26 (d. 26), were amplified by PCR, and the cDNA transcripts then cloned and sequenced, separately for each mouse. The predicted amino acid sequence encoded by each unique junction is shown (which includes the C-terminal portion of Vγ4, N-encoded amino acids, and N-terminal Jγ1 amino acids), and the number of clones obtained represented by each sequence is indicated; the conserved motif (top line) was predominant. B. The number of Vγ4Vδ4+ cells obtained from DBA/1LacJ mice treated as indicated; some mice were immunized only once (1X) and the lymph node cells harvested as indicated on day 9 or day 11, whereas others were injected twice (2X) on days 0 and 21 and the lymph node cells harvested on day 26. Each group contained 3–9 mice; each symbol within a group represents the result from an individual mouse; error bars show the s.d. C. As for B, except the average percent of Vγ4+ cells co-expressing Vδ4 is indicated for each treatment group.