Fig. 2. C57BL/6 mice immunized with CFA also causes preferential expansion of the canonical Vγ4Vδ4+ subset in the draining lymph nodes.
A. The number of Vγ4Vδ4+ cells obtained from the lymph nodes of individual C57BL/6 mice immunized with intradermal CFA on days 0 and 21 and sacrificed on day 26, or immunized on days 0 and 14 and sacrificed on day 19, or immunized on day 0 and sacrificed on day 9, are shown. B. As for A, except the average percent of Vγ4+ cells co-expressing Vδ4 is indicated for each treatment group. C. Four C57BL/6 male mice were treated as for Fig. 1A, except that mice were immunized with CFA/collagen (the CIA protocol) instead of CFA only, and cloned Vγ4 transcripts from each individual were sequenced.