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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Immunol Res. 2013 Mar;55(1-3):217–230. doi: 10.1007/s12026-012-8364-9

Fig. 9. The αβ T cell response to intradermal CFA is altered when Vγ4+ cells are inactivated.

Fig. 9

C57BL/6 mice were immunized intradermally with CFA on days 0 and 21, with intravenous injections of anti-Vγ4 antibody preceding each immunization by 4 days; lymph nodes were harvested on day 26. A–B: Each group represents the average obtained from 4 mice whose cells were analyzed individually, except the naïve control, included for comparison, for which cells from the lymph nodes of 4 mice were pooled together before the analysis. A. Numbers of αβ T cells obtained expressing IL-17A intracellularly after 4 hours of culture with PMA/ionomycin. B. As for A, except the percent of αβ T cells expressing the indicated cytokine is shown. C. The percent of IL-17A+ cells present in CD4+ cells was determined. Here, mice were immunized by intradermal injection of CFA on day 0 and day 14, and the lymph nodes harvested on d. 19. Error bars show the range obtained from samples from duplicate mice analyzed separately; for naïve TCRδ−/− and naive Vγ4/6−/− mice, shown for comparison, the result was determined from cells obtained from a single mouse only.