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. 2012 Nov 6;304(1):E74–E86. doi: 10.1152/ajpendo.00176.2012

Fig. 9.

Fig. 9.

Effect of the lysosomal inhibitor bafilomycin A1 on LT (4°C, 3 wk)-induced change in autophagy and cardiomyocyte contractile properties. Cardiomyocytes isolated from NT- or LT-maintained FVB mice were incubated with the autophagic flux inhibitor bafilomycin (100 nM) for 4 h prior to assessment of autophagy and cardiomyocyte mechanics. A: LC3B-II level. B: LC3B-II/LC3B-I ratio. C: p62 level. A and C, top: representative gel blots depicting autophagy markers (GAPDH as loading control) using specific antibodies. D: resting cell length. E: peak shortening. F: +dl/dt. G: −dl/dt. H: TPS. I: TR90. Data are means ± SE; n = 4 isolations (A–C) or 105–107 cells from 4 mice/group (D–I). *P < 0.05 vs. FVB-NT group; #P < 0.05 vs. FVB-LT group.