Fig. 2.

NPY does not affect, whereas SST inhibits, Ca²⁺ activity. Changes in Fluo 4-AM signal recorded with and without NPY (100 nM) or SST (1 μM) at 10 mM glucose to detect frequency and amplitude of [Ca²⁺]_i oscillations. A and C: representative oscillations in [Ca²⁺]_i recorded from intact islets before (dotted line) and after (solid line) treatment with NPY (A) or SST (C). B and D: normalized frequency of [Ca²⁺]_i oscillations pre- and posttreatment with NPY (B) or SST (D). Data prior to addition of NPY or SST are shown in white; data after addition of the ligand are represented in gray (NPY) or black (SST). Data collected following treatment with the ligand are normalized to the data collected from the islet prior to addition of the ligand. Data are means ± SE; n = 6–7. *P < 0.04.