Fig. 5.

SST abolishes the mSIRK-induced increase in [Ca²⁺]_i oscillation frequency, whereas NPY does not; neither effect [Ca²⁺]_i with gallein. Changes in Fluo 4-AM signal recorded at 10 mM glucose with and without gallein (10 μM) or mSIRK (30 μM) and NPY (100 nM) or SST (1 μM). A and C: representative [Ca²⁺]_i oscillations recorded from intact islets before (dotted line) and after (solid line) treatment with NPY and gallein (A) or mSIRK (C). B and D: normalized frequency of [Ca²⁺]_i oscillations measured before (white) and after treatment with NPY and gallein (B, gray checkered) or mSIRK (D, gray striped). E and G: representative [Ca²⁺]_i oscillations from intact islets recorded pre- (dotted line) and posttreatment (solid line) with SST and gallein (E) or mSIRK (G). F and H: normalized [Ca²⁺]_i oscillation frequency pre- (white) and posttreatment with SST and gallein (F, black checkered) or mSIRK (H, black striped). Data are means ± SE; n = 3–4. *P < 0.05.