Fig. 6.
NPY, SST, and gallein do not alter [Ca2+]i at low glucose, but mSIRK stimulates [Ca2+]i release. Changes in Fluo 4-AM signal recorded at 2 mM glucose with and without NPY (100 nM), SST (1 μM), gallein (10 μM), mSIRK (30 μM), or a combination. A: Fluo 4-AM fluorescence intensity normalized to untreated control (white) before and after treatment with NPY (gray), gallein (white checkered), NPY and gallein (gray checkered), mSIRK (white striped), or NPY and mSIRK (gray striped). B: normalized Fluo 4-AM fluorescence intensity pre- (white) and posttreatment with SST (black), gallein (black checkered), SST and gallein (black checkered), mSIRK (white striped), or SST and mSIRK (black striped). Data are means ± SE; n = 5. *P < 0.05; #P < 0.005.