Table 1.
Measured [Ca2+]i oscillation parameters, as detected by Fluo 4-AM imaging
| GPCR Ligand/Gβγ Modulator | n | GPCR Ligand/Gβγ Modulator | Frequency, mHz | Amplitude, %Δ |
|---|---|---|---|---|
| Neuropeptide Y | 6 | − | 36.33 ± 1.7 | 9.45 ± 2.0 |
| 6 | + | 40.44 ± 2.2 | 10.04 ± 2.3 | |
| Somatostatin | 7 | − | 34.47 ± 1.9 | 7.05 ± 1.6 |
| 7 | + | 27.16 ± 2.9* | 8.74 ± 1.0 | |
| Gallein | 5 | − | 44.78 ± 7.8 | 4.23 ± 3.9 |
| 5 | + | 41.17 ± 7.9 | 1.32 ± 1.2 | |
| mSIRK | 5 | − | 37.13 ± 7.3 | 16.82 ± 6.1 |
| 5 | + | 57.83 ± 8.2* | 7.10 ± 2.2 | |
| NPY + gallein | 3 | − | 20.54 ± 3.2 | 21.05 ± 3.4 |
| 3 | + | 25.49 ± 9.4 | 17.88 ± 6.1 | |
| NPY + mSIRK | 4 | − | 20.65 ± 2.8 | 13.79 ± 1.7 |
| 4 | + | 32.60 ± 4.8* | 5.01 ± 0.6* | |
| SST + gallein | 3 | − | 18.98 ± 1.9 | 17.49 ± 4.3 |
| 3 | + | 16.58 ± 5.0 | 15.61 ± 2.9 | |
| SST + mSIRK | 4 | − | 30.68 ± 3.1 | 9.76 ± 5.7 |
| 4 | + | 26.57 ± 5.2 | 11.13 ± 7.0 |
Data are means ± SE for frequency and amplitude of [Ca2+]i oscillations measured pre- and posttreatment with the specified G protein-coupled receptor (GPCR) ligand and/or Gβγ modulator. Significance was tested using a two-tailed Student's t-test to compare the frequency or amplitude between untreated and treated islets. Significance P< 0.04.