Fig. 3.

Effect of selective ligands of type 1 and type 2 receptors on morphology of preadipocytes from SC rat adipose tissue in primary culture. Preadipocytes were plated in 35-mm Petri dishes at a density of 1.5 × 10⁴/cm² and cultured for 7 days as described in research design and methods in the absence (control) or presence of ANG II (100 nM) or C21/M24 (10 nM) alone or in combination with AT1R antagonist losartan (LOS; 1 μM) or AT2R antagonist PD123,319 (PD; 1 μM). A: histograms illustrating percentage of cell types exhibiting small lipid droplets (LD). B: analyses of mean diameter of small LD. Values were obtained from data analyses (histograms and fitting curves) constructed as described in research design and methods and in Supplemental Data. Data are means ± SE of LD from 36 images taken from 3 different Petri dishes from 2 different experiments. Results include mean values obtained at day 7 in culture, thus n = 24. Statistical analyses were performed using two-way ANOVA followed by Bonferroni multiple comparisons. Statistical significance vs. respective control: ***P < 0.001. C: preadipocytes were plated in 35-mm Petri dishes at a density of 1.5 × 10⁴/cm² and cultured for 7 days in the absence (control) or presence of ANG II (100 nM), or C21/M24 (10 nM) alone or in combination with losartan (1 μM) or PD123,319 (1 μM). Scale bars, 40 μm.