Fig. 3.

Generation of transgenic mice expressing stabilized versions of hypoxia-inducible factor (HIF)1A or HIF2A subunits in podocytes. A: targeting to generate a stabilized version of HIF1A and HIF1B. B: breeding strategies to generate mice carrying stabilized HIF1 α or HIF2 α mutant alleles in their podocytes. C: Western blot analysis of HIF1A and HIF 2A levels in glomeruli isolated from transgenic mice, detected by antibodies to the HA tag. Only mice homozygous for the HIF2a transgene show significantly elevated protein levels on Western blot.