Skip to main content
. 2012 Nov 7;304(2):F177–F188. doi: 10.1152/ajprenal.00431.2012

Fig. 3.

Fig. 3.

dDAVP significantly reduces levels of the cleaved forms of caspase-3, caspase-7, and PARP in cells treated with a variety of proapoptotic agents. MpkCCD cells were incubated in the absence (−) or presence (+) of 0.1 nM dDAVP for 4 h. Actinomycin D (10 μM), camptothecin (2 μM), dexamethasone (10 μM), cycloheximide (100 μM), etoposide (100 μM), or DMSO vehicle was added 15 min after the start of dDAVP treatment. A: representative immunoblots show the levels of the cleaved forms of caspase-3 (17 kDa), caspase-7 (20 kDa), and PARP (89 kDa), as well as full-length PARP (166 kDa), and actin (45 kDa) as a loading control. Also shown is quantification of band densities from replicate immunoblots of the cleaved forms of caspase-3 (B), caspase-7 (C), and PARP (bottom band; D) from cells treated with actinomycin D, cycloheximide, or DMSO vehicle. P values were calculated using paired ANOVA and a Bonferroni posttest. ***P < 0.001. *P < 0.05.