Fig. 4.

V2 receptor antagonist SR121463 blocks the reduction in cleaved apoptotic proteins in response to dDAVP. MpkCCD cells were preincubated for 15 min with SR121463 (0.01 or 1 μM) or vehicle control, followed by incubation in the absence (−) or presence (+) of 0.1 nM dDAVP for 2 h. SS or DMSO control was added 15 min after the start of dDAVP treatment. A: representative immunoblots show the levels of the cleaved forms of caspase-3, caspase-7, and PARP (bottom band). B: quantification of band densities from replicate immunoblots of the cleaved forms of caspase-3, caspase-7, and PARP. P values were calculated using paired t-tests. *P < 0.05.