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. 2012 Nov 7;304(2):F177–F188. doi: 10.1152/ajprenal.00431.2012

Fig. 7.

Fig. 7.

Phosphatidylinositol 3-kinase (PI3-kinase) inhibitor LY294002 inhibits Akt phosphorylation and increases apoptosis. MpkCCD cells were preincubated for 15 min with LY294002 (25 or 50 μM) or vehicle control, followed by incubation in the absence (−) or presence (+) of 0.1 nM dDAVP for 2 h. SS or DMSO control was added 15 min after the start of dDAVP treatment. A: representative immunoblots show the levels Akt phosphorylated at Thr-308 (Akt-pT308), Akt phosphorylated at Ser-473 (Akt-pS473), total Akt (Akt), cleaved caspase-3, cleaved caspase-7, cleaved PARP (bottom band), and full-length PARP (top band). B: quantification of band densities from replicate immunoblots of both forms of phosphorylated Akt. C: quantification of band densities from replicate immunoblots of the cleaved forms of caspase-3, caspase-7, and PARP. P values were calculated using paired ANOVA and a Bonferroni posttest. ***P < 0.001. *P < 0.05.