Fig. 8.

Phosphorylation of Bcl-2 family member proteins is regulated by dDAVP and cAMP, independently of PI3K. A: mpkCCD cells were incubated with either dDAVP (0.1 nM), 8-cpt-cAMP (100 μM), Me-cAMP (100 μM), or vehicle control for 30 min followed by immunoblotting for phosphorylated as well as total levels of Bad, Bok, and Akt. Cells preincubated with either SR121463 (1 μM) or LY294002 (50 μM) before dDAVP treatment were also included (dDAVP+SR and dDAVP+LY, respectively). B: quantification of band densities from replicate immunoblots probed for Bad phosphorylated at Ser-112 (Bad-pS112), Bad phosphorylated at Ser-155 (Bad-pS155), and Bok phosphorylated at Ser-8 (Bok-pS8). C: quantification of Akt phosphorylated at Thr-308 (Akt-pT308) and Ser-473 (Akt-pS473). P values were calculated using paired ANOVA followed by Dunnett's posttest. *, **, and ***: P < 0.05, <0.01, and <0.001, respectively, vs. vehicle control.