Fig. A1.

Characterization of a newly generated phospho-specific Bok antibody. A: a rabbit polyclonal antibody recognizing Bok phosphorylated at Ser-8 was tested for reactivity with the immunizing phosphopeptide (lane 1), the unphosphorylated version of the same peptide (lane 2), and a peptide corresponding to the phosphorylated C terminus of aquaporin-2 (AQP2-pS256) as a negative control (lane 3). B: rat collecting duct samples incubated in the absence (−) or presence (+) of 1 nM dDAVP were probed with the phospho-specific Bok antibody preadsorbed with either the unphosphorylated Bok antigenic peptide (top) or the phosphorylated Bok antigenic peptide (bottom).