Table 2.
Significant changes in protein abundance during chronic metabolic acidosis
| Uniprot |
Mean Spectral Counts ± SE |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Protein Name | Accession No. | Gene | Mass, kDa | P Value | Control | 7-Day CMA | Fold-Change | Potential pH-RE | Location | TMD |
| UDP-glucuronosyltransferase 2B15 | P36511 | Ugt2b15 | 61 | 0.00022 | 1.8 ± 1.0 | 17.0 ± 0.7 | 6.4 | Yes | ER | 1 |
| Carbonic anhydrase 5B, mitochondrial | Q66HG6 | Ca5b | 37 | 0.068* | 0.0 ± 0.0 | 4.7 ± 1.9 | 5.7 | Yes | M | |
| Protein AMBP | Q64240 | Ambp | 39 | 0.024 | 2.0 ± 0.9 | 13.8 ± 3.2 | 5.0 | No | Membrane | |
| UDP-glucuronosyltransferase 1-1 | Q64550 | Ugt1a1 | 60 | 0.00086 | 11.0 ± 1.8 | 54.1 ± 4.5 | 4.6 | Yes | ER | 2 |
| All-trans-13,14-dihydroretinol saturase, CRA_b | G3V7V6 | Retsat | 67 | 0.043 | 0.7 ± 0.4 | 7.5 ± 2.3 | 4.9 | No | Unknown | 1 |
| Ras-related protein Rab-21 | Q6AXT5 | Rab21 | 24 | 0.011 | 1.5 ± 0.8 | 9.5 ± 1.6 | 4.1 | Yes | Membrane | |
| Uncharacterized protein | D4A0Y1 | Cfb | 141 | 0.014 | 1.2 ± 0.7 | 7.7 ± 1.4 | 4.0 | NA | Extracellular | |
| Glutaminase kidney isoform, mitochondrial | P13264 | Gls | 74 | 0.019 | 17.7 ± 2.1 | 59.3 ± 10.7 | 3.2 | Yes | M | |
| Enoyl-CoA hydratase domain-containing protein 3 | Q3MIE0 | Echdc3 | 32 | 0.0061 | 0.4 ± 0.4 | 3.8 ± 0.6 | 3.6 | Yes | M | |
| Epoxide hydrolase 1 | P07687 | Ephx1 | 53 | 0.013 | 11.1 ± 3.3 | 36.3 ± 4.9 | 3.1 | No | ER | |
| Vitamin D-binding protein | P04276 | Gc | 54 | 0.0062 | 15.0 ± 4.4 | 40.2 ± 1.9 | 2.6 | No | Extracellular | |
| Acetyl-coenzyme A acyltransferase 1B | F1LPD6 | Acaa1b | 44 | 0.0049 | 11.3 ± 1.1 | 28.4 ± 2.8 | 2.4 | No | M and P | |
| Catalase | P04762 | Cat | 60 | 0.0095 | 55.1 ± 12.0 | 123.1 ± 8.3 | 2.2 | Yes | M and P | |
| Ribonuclease 4 | O55004 | Rnase4 | 17 | 0.044 | 7.5 ± 1.4 | 17.7 ± 3.2 | 2.2 | Yes | Extracellular | 1 |
| Ectonucleoside triphosphate diphosphohydrolase 5 | Q6P6S9 | Entpd5 | 47 | 0.018 | 8.0 ± 0.8 | 18.2 ± 2.5 | 2.1 | No | ER | 2 |
| Enoyl-coenzyme A hydratase/3-hydroxyacyl-coenzyme A | P07896 | Ehhadh | 79 | 0.014 | 14.3 ± 3.5 | 31.5 ± 2.0 | 2.1 | Yes | M and P | |
| Fumarylacetoacetate hydrolase domain-containing 2 | B2RYW9 | Fahd2 | 35 | 0.011 | 29.6 ± 5.9 | 61.1 ± 3.8 | 2.0 | Yes | M | |
| 17-β-Hydroxysteroid dehydrogenase 4 | P97852 | Hsd17b4 | 79 | 0.0018 | 48.1 ± 3.6 | 95.3 ± 5.3 | 2.0 | No | M and P | |
| Cytochrome P-450 4A2 | P20816 | Cyp4a2 | 58 | 0.023 | 91.2 ± 22.0 | 175.1 ± 7.7 | 1.9 | Yes | ER | 1 |
| Peroxisomal acyl-coenzyme A oxidase 1 | P07872 | Acox1 | 75 | 0.021 | 26.2 ± 6.8 | 51.4 ± 0.5 | 1.9 | No | ER and M | |
| Dimethylglycine dehydrogenase, mitochondrial | Q63342 | Dmgdh | 96 | 0.0024 | 47.4 ± 7.6 | 68.3 ± 2.5 | 1.5 | Yes | M | |
| Apolipoprotein A-IV | P02651 | Apoa4 | 44 | 0.041 | 46.3 ± 10.0 | 83.6 ± 7.6 | 1.8 | No | ER | |
| ATP-binding cassette subfamily D member 3 | P16970 | Abcd3 | 75 | 0.037 | 29.3 ± 6.3 | 52.5 ± 4.0 | 1.8 | Yes | M and P | 3 |
| Cytochrome b5 | P00173 | Cyb5a | 15 | 0.014 | 37.5 ± 5.8 | 64.7 ± 4.8 | 1.7 | Yes | ER and M | 1 |
| Glutamate dehydrogenase 1, mitochondrial | P10860 | Glud1 | 61 | 0.0035 | 431.5 ± 45.2 | 715.7 ± 7.9 | 1.7 | Yes | M | |
| Probable d-lactate dehydrogenase, mitochondrial | F1LVD7 | Ldhd | 52 | 0.037 | 27.0 ± 4.0 | 45.7 ± 4.5 | 1.7 | NA | M | |
| NADPH-cytochrome P-450 reductase | P00388 | Por | 77 | 0.044 | 13.2 ± 1.4 | 21.6 ± 2.6 | 1.6 | Yes | ER and M | 1 |
| GrpE protein homolog 1, mitochondrial | P97576 | Grpel1 | 24 | 0.04 | 10.2 ± 0.9 | 16.3 ± 1.8 | 1.5 | No | M | |
| Enoyl-CoA δ isomerase 1, mitochondrial | P23965 | Eci1 | 32 | 0.035 | 76.7 ± 6.5 | 116.2 ± 10.8 | 1.5 | No | M | |
| Filamin-B | D3ZD13 | Flnb | 278 | 0.023 | 4.2 ± 0.5 | 2.1 ± 0.3 | 0.6 | NA | Cytosol | |
| 4F2 cell-surface antigen heavy chain | Q794F9 | Slc3a2 | 58 | 0.02 | 105.6 ± 13.0 | 50.5 ± 6.8 | 0.5 | NA | Membrane | 1 |
| Calnexin | P35565 | Canx | 67 | 0.0069 | 35.1 ± 3.6 | 14.8 ± 1.5 | 0.4 | NA | ER | 1 |
| Protein FAM151A | Q642A7 | Fam151a | 67 | 0.036 | 29.0 ± 6.1 | 13.0 ± 2.6 | 0.4 | NA | Membrane | 1 |
Proteins in italics were previously shown to be induced during acidosis.
*
Greater than 0.05 P value. pH-RE, potential pH response element; NA if 3′−UTR not sequenced or fold-decrease; M, location mitochondria; P, peroxisome; ER, endoplasmic reticulum; TMD, no. of transmembrane domains predicted.