Fig. 8.

Dual fluorescence staining for 5-ethynyl-2′-deoxyuridine (EdU) and basal cell marker keratin 5 (K5). FVB/NJ and A/J mice were exposed to chlorine, and lung sections collected from mice 1, 4, and 7 days after exposure were analyzed by dual staining. Nuclei in proliferating cells are stained green (EdU staining) and K5 is stained red. Tissues were counterstained with DAPI (blue). Bar in H represents 20 μm for all panels.