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Metformin does not affect protein synthesis factors in IPH-PAEC. IPH-PAEC was treated with Metformin (5 × 10−6, 5 × 10−5, and 5 × 10−4 M) for 24 h. The phosphorylation of Akt, eukaryotic initiation factors-4E (eIF4E), and S6 did not change after treatment. Phosphorylation of P70S6K-2 increased with Metformin at 5 × 10−6 and 5 × 10−5 M but was not associated with increased S6 phosphorylation.
