Group mean changes in contractile properties in single myofibers during the stimulation protocol expressed in cold (C, 15°C) and hot (H, 25°C). All values are expressed relative to the starting normal time point (N, 20°C): relative tension (A), tension-time integral (TTI) (B), estimated ATP turnover (MRR × peak tension) (C), and estimated relative economy (estimated as TTI/(MRR × peak tension); reflecting the ratio of mechanical output to total energy consumption) (D). *P < 0.05 compared with N. +P < 0.05 compared the previous time point. Values are means ± SE.