Inhibitory effects of SKA-31 on carbachol-induced contractions in human DSM-isolated strips. A: representative recording from a DSM-isolated strip demonstrating the inhibitory effects of SKA-31 (10 μM) on 0.1 μM carbachol-induced contractions. B: summary data and statistical analyses showing the inhibitory effects of SKA-31 on the carbachol-induced phasic contraction amplitude, muscle integral force, contraction duration, contraction frequency, and muscle tone in human DSM-isolated strips (n = 5, N = 5; ***P < 0.005; **P < 0.01 vs. control). All experiments were performed in the presence of 1 μM TTX.