Fig. 2.

Lymphatics and nerves remain present in the rat mesentery culture model in serum free media for 3 days. A–C: lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1) and PECAM immunolabeling identified intact lymphatic networks (arrows) after 3 days of culture in serum free media. D–F: class III β-tubulin and PECAM immunolabeling. After 3 days of culture in serum free media. Positive class III β-tubulin immunolabeling identified nerves (arrows). Scale bars = 200 μm (A–C), 25 μm (D–F).