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Microvascular networks can be stimulated to undergo angiogenesis while in culture for 3 days. Networks after 3 day culture in serum-free MEM (A), MEM plus bFGF (B), MEM plus VEGF (C), and MEM plus bFGF and NG2 antibody (D). E and F: higher magnification images of capillary sprouts (arrows) off a venule and existing capillaries. “A” indicates arteriole. “V” indicates venule. “c” indicates capillary. Scale bars = 200 μm (A–D), 50 μm (E and F).
