Table 1.

Overview of myofilament protein phosphorylation before and after exchange

	Cardiac Myosin-Binding Protein-C	Desmin	Myosin Light Chain 2
Before exchange	0.60 ± 0.07	0.27 ± 0.02	0.25 ± 0.04
Control exchange	0.58 ± 0.07	0.26 ± 0.03	0.31 ± 0.01
cTnI-AA	0.56 ± 0.04	0.24 ± 0.06	0.31 ± 0.03
cTnI-AD	0.54 ± 0.05	0.24 ± 0.05	0.28 ± 0.02
cTnI-DA	0.58 ± 0.11	0.29 ± 0.04	0.27 ± 0.02
cTnI-DD	0.55 ± 0.03	0.23 ± 0.06	0.25 ± 0.02

Values are means ± SE of the ProQ/SYPRO intensity ratio. Shown is myofilament protein phosphorylation determined before and after exchange in three donor samples via ProQ diamond staining. Control exchange cardiomyocytes were kept overnight in exchange solution without recombinant cardiac troponin (cTn)I added. No significant effect was found of the control exchange protocol on myofilament protein phosphorylation compared with before exchange (by Student's t-test). cTn exchange at 1 mg/ml also did not affect myofilament protein phosphorylation (by one-way ANOVA comparing control exchange and the four cTnI exchange groups). A, alanine substitution; D, aspartic acid substitution.