Table 1.
Lipid Manipulated |
Enzymatic Tool | Targeted Membrane/Compartment; Targeting Sequence/Protein |
Dimerizer Used | Phenotypes | Reference |
---|---|---|---|---|---|
PI(4,5)P2 | type IV 5-phosphatase domain | PM; palmitoylation sequence of GAP43 | rapamycin, AP21967 | termination of ATP-induced Ca2+ signal, inactivation of TRPM8 channels, blockade of TfnR and EGFR endocytosis;, | (Varnai et al., 2006) |
PI(4,5)P2 | Inp54p phosphatase | PM; Lyn11 | iRAP | current of KCNQ ion channels fall to 0 | (Suh et al., 2006) |
PI(4,5)P2 | Inp54p phosphatase | PM; Lyn11 | iRAP | with simultaneous blockade of PIP3 production, proteins with polybasic clusters translocated from PM | (Heo et al., 2006) |
PI3P | myotubularin 1 | endosomes; Rab5a | AP21967 | TfnR accumulation in Rab5a-positive endosomes, endosome positive tubularization | (Fili et al., 2006) |
PI(4,5)P2 | type IV 5-phosphatase domain | PM; palmitoylation sequence of GAP43 | iRAP | loss of endocytic CCPs, dissociation of endocytic adaptors, dynamin and Arp2/3 complex from PM | (Zoncu et al., 2007) |
PI(4,5)P2 | Inp54p phosphatase domain | PM; Lyn11 | iRAP | decreased TfnR endocytosis, increased TfnR concentration on PM, dissociation of AP-2 from PM | (Abe et al., 2008) |
PI(4,5)P2 | p110 catalytic subunit of type I PI 3-kinase | PM; palmitoylation sequence of GAP43 | iRAP | PIP3 production, membrane ruffles, accelerated CCP maturation and turnover | (Nakatsu et al., 2010) |
PI(4,5)P2 | 5-phosphatase domain of Synj1 | PM; endophilin N-BAR domain | rapamycin, AP21967 | membrane tubule fission | (Chang-Ileto et al., 2011) |
CCP: clathrin-coated pit; EGFR: epidermal growth factor receptor; PM: plasma membrane; Synj1: synaptojanin 1; Tfn: transferrin; TfnR: transferrin receptor; TRPM8: methanol-activated transient receptor potential melastatin 8.