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. Author manuscript; available in PMC: 2013 Jan 12.
Published in final edited form as: Methods Cell Biol. 2012;108:187–207. doi: 10.1016/B978-0-12-386487-1.00010-9

Table 1.

Examples in the literature of CID use to manipulate phosphoinostide levels.

Lipid
Manipulated
Enzymatic Tool Targeted
Membrane/Compartment;
Targeting
Sequence/Protein
Dimerizer Used Phenotypes Reference
PI(4,5)P2 type IV 5-phosphatase domain PM; palmitoylation sequence of GAP43 rapamycin, AP21967 termination of ATP-induced Ca2+ signal, inactivation of TRPM8 channels, blockade of TfnR and EGFR endocytosis;, (Varnai et al., 2006)
PI(4,5)P2 Inp54p phosphatase PM; Lyn11 iRAP current of KCNQ ion channels fall to 0 (Suh et al., 2006)
PI(4,5)P2 Inp54p phosphatase PM; Lyn11 iRAP with simultaneous blockade of PIP3 production, proteins with polybasic clusters translocated from PM (Heo et al., 2006)
PI3P myotubularin 1 endosomes; Rab5a AP21967 TfnR accumulation in Rab5a-positive endosomes, endosome positive tubularization (Fili et al., 2006)
PI(4,5)P2 type IV 5-phosphatase domain PM; palmitoylation sequence of GAP43 iRAP loss of endocytic CCPs, dissociation of endocytic adaptors, dynamin and Arp2/3 complex from PM (Zoncu et al., 2007)
PI(4,5)P2 Inp54p phosphatase domain PM; Lyn11 iRAP decreased TfnR endocytosis, increased TfnR concentration on PM, dissociation of AP-2 from PM (Abe et al., 2008)
PI(4,5)P2 p110 catalytic subunit of type I PI 3-kinase PM; palmitoylation sequence of GAP43 iRAP PIP3 production, membrane ruffles, accelerated CCP maturation and turnover (Nakatsu et al., 2010)
PI(4,5)P2 5-phosphatase domain of Synj1 PM; endophilin N-BAR domain rapamycin, AP21967 membrane tubule fission (Chang-Ileto et al., 2011)

CCP: clathrin-coated pit; EGFR: epidermal growth factor receptor; PM: plasma membrane; Synj1: synaptojanin 1; Tfn: transferrin; TfnR: transferrin receptor; TRPM8: methanol-activated transient receptor potential melastatin 8.