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. 2013 Jan 3;13:3. doi: 10.1186/1471-2229-13-3

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Copyright ©2013 Hörnblad et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Knockout of the Physcomitrella PpGT47A gene does not cause a significant change in cell wall monosaccharide composition.A) Schematic drawing of the PpGT47A gene and the knock-out construct which was introduced via homologous recombination to obtain knockout mutants in Physcomitrella. ** corresponds to a truncated version of the E4 sequence and * corresponds to a truncated version of the 3^′UTR sequence which are indicated in the upper genomic sequence. B) Monosaccharide analysis of gametophore cell walls from Physcomitrella wild-type and 4 independent gt47a knockout mutants. Data were obtained from 3 technical replicates of two pooled samples for the wild-type and knockout Ppgt47a:3, and three pooled samples for knockouts Ppgt47a:2; Ppgt47a:5 and Ppgt47a:11 .