Table 2.
Phobius transmembrane and signal sequence prediction for members of the Arabidopsis, Populus and Physcomitrella IRX10 family of proteins
| Protein | Amino acid | Prediction | Protein | Amino acid | Prediction |
|---|---|---|---|---|---|
|
AtIRX10 |
1-21 |
Signal |
AtIRX10-L |
1-19 |
Signal |
| |
1-5 |
N-region |
|
1-2 |
N-region |
| |
6-16 |
H-region |
|
3-13 |
H-region |
| |
17-21 |
C-region |
|
14-21 |
C-region |
| |
22-412 |
Non-cytoplasmic |
|
22-415 |
Non-cytoplasmic |
|
PtGT47A-1 |
1-19 |
Signal |
PtGT47A-2 |
1-19 |
Signal |
| |
1-2 |
N-region |
|
1-2 |
N-region |
| |
3-14 |
H-region |
|
3-14 |
H-region |
| |
15-19 |
C-region |
|
15-19 |
C-region |
| |
20-412 |
Non-cytoplasmic |
|
20-413 |
Non-cytoplasmic |
|
PtGT47D-1 and PtGT47D-4 |
1-5 |
Cytoplasmic |
PpGT47A |
1-31 |
Cytoplasmic |
| |
6-26 |
Transmembrane |
|
32-51 |
Transmembrane |
| 27-417 | Cytoplasmic | 52-449 | Non-cytoplasmic |
IRX10 related protein sequences from Arabidopsis, Populus and Physcomitrella were analysed using the Phobius programme (http://www.phobius.sbc.su.se) for the presence of N-terminal signal sequences and the locations of the hydrophobic predicted transmembrane core (H-region) flanked by the N- and C- regions.