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Dual-immunofluorescence detection of sirtuin-1 (Sirt1) (red) and alpha-actin (green) (a–d) and of endothelin-1 (ET1) (red) and endothelial nitric oxide synthase (eNOS) (green) (e–h) in young (a, e), androgen-deprived (b, f), and aged (c, g) human cavernous tissue. The nucleus is stained blue (DAPI). Negative controls were performed by primary antibody omission (d, h)
