Ethanol treatment produces increasing blood ethanol concentrations in postnatal day 6 rat pups. Animals are treated with 0, 4.5, 5.25 or 6.0 g/kg/d of ethanol, delivered in two feedings, 2 hours apart. Blood is obtained 2 hours after the second feeding from a tail clip. Results shown are from a male and female pair from each experiment averaged from 3 separate experiments. Blood ethanol concentrations are expressed as mg/dL. The bar indicates the mean values +/- SD of six pups. ANOVA single factor, p<10-10; *vs both SC and IC, p<0.0001, paired t-test.