Figure 6.
Dynamic cell spreading. (A) High magnification images of HUVECs and hMSCs on 5% RGD at 24 and 72 hrs (vinculin:green, actin:red, and nuclei:blue). Quantification of projected cell areas between cell types at (B) 24 and (C) 72 hrs as well as comparison within (D) HUVECs and (E) hMSCs at both time points. (F) Relative spreading was determined by scaling projected cell area to maximum projected areas for each cell type and time point. (Error bars indicate standard error of the mean. Asterisk indicates significant increase and cross indicates significant decrease in projected cell area at the indicated GRGDSP density, p < 0.05.)