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Apoptotic induction in either (a) OST cells or in (b) LM8 cells after adding ESA. The cells were cultured in 10% FBS D-MEM with 50 μg/mL ESA (bottom panel). As control, only PBS (no ESA) was added (top panel). The cells were incubated with ESA for either 3 hours or 24 hours. Induction of apoptosis in these cells was detected by means of the double staining assay for annexin V-PE and 7-ADD.
