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Determination of the caspase-3 activity of OST cells treated with ESA. The OST cells were cultured during 16 hours in D-MEM containing either a solution of 10% FBS and 50 μg/mL ESA in PBS or a solution of 50 μg/mL ESA and ZVAD-FMK (as caspase inhibitor) in PBS. Caspase-3 activity was determined from the absorbance values measured at 405 nm as “activity index” by use of a spectrophotometer. The values are means and standard deviations for three separate measurements.
