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. Author manuscript; available in PMC: 2013 Jan 13.
Published in final edited form as: J Neural Eng. 2012 Nov 27;9(6):065001. doi: 10.1088/1741-2560/9/6/065001

Figure 3. βIII tubulin labeling of stem cells in vitro and in vivo.

Figure 3

Immunofluorescence photomicrographs of differentiated stem cells embedded in hydrogel in vitro (A,B) and in vivo (C, D), expressing green fluorescence protein (GFP, green), βIII tubulin (BIII, red) and counterstained with the nuclear marker DAPI (blue). In vitro preparations (A,B) illustrate the variable expression of GFP within this cell line (low expression, arrowheads; high expression, arrows). Low magnification image in (C) showing location of stem cells in the cochlea, and higher magnification of inset in (D) showing immunopositive labeling. Scale bars = 20 μm (A,B,D); 50 μm (C).