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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2012 Oct 24;529(1):18–25. doi: 10.1016/j.abb.2012.10.007

Figure 3. Cleavage of hexa-His tag from purified rat apoE.

Figure 3

Following purification, 4–20% acrylamide gradient SDS-PAGE was performed of recombinant rat apoE (10 μg) that was treated with 3U of AcTEV™ protease at 24 °C (left) or 30 °C (right) for 0, 1, 2, 3 or 4 h. Following incubation, the reaction was stopped by addition of SDS-PAGE sample treatment buffer and the samples directly electrophoresced on a 4–20% acrylamide gradient SDS-PAGE gel.