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. Author manuscript; available in PMC: 2014 Jan 1.

Published in final edited form as: Arch Biochem Biophys. 2012 Oct 24;529(1):18–25. doi: 10.1016/j.abb.2012.10.007

A. Far-UV CD spectra of apoE. The spectra of rat (solid line) and human apoE3 (dashed line) (0.2 mg/ml) were recorded in 20 mM sodium phosphate buffer, pH 7.4. The spectra were recorded from 185 to 260 nm using a 0.1 cm path length cell, scan speed of 20 nm per minute, and response time of 1 second (average of 3 scans shown). B. GdnHCl-induced unfolding of apoE. The % maximal change in ellipticity of 0.2 mg/ml rat apoE (closed circles) and human apoE3 (open circles) at 222 nm was plotted as a function of increasing GdnHCl concentration. ApoE3 was pre-incubated with 5-fold molar excess of reducing agent, dithiothreitol, to reduce existing disulfide bonds.

A. Far-UV CD spectra of apoE. The spectra of rat (solid line) and human apoE3 (dashed line) (0.2 mg/ml) were recorded in 20 mM sodium phosphate buffer, pH 7.4. The spectra were recorded from 185 to 260 nm using a 0.1 cm path length cell, scan speed of 20 nm per minute, and response time of 1 second (average of 3 scans shown). B. GdnHCl-induced unfolding of apoE. The % maximal change in ellipticity of 0.2 mg/ml rat apoE (closed circles) and human apoE3 (open circles) at 222 nm was plotted as a function of increasing GdnHCl concentration. ApoE3 was pre-incubated with 5-fold molar excess of reducing agent, dithiothreitol, to reduce existing disulfide bonds.