Table 1.
Comparison of biochemical composition measurements of freshly isolated immature and mature cartilage, and, in vitro cultured (ITS) and growth factor-treated (ITS-FGF2-TGFβ1) immature articular cartilages.
| Immature AC | Mature AC | Immature AC |
||
|---|---|---|---|---|
| ITS | ITS-FGF2-TGFβ1 | |||
| % water (mg)/wet weight (mg) | 73.8 ± 1.07 | 68.7 ± 1.67a | 77.7 ± 1.70 | 68.7 ± 2.54ba |
| dry/wet weight ratio | 0.26 ± 0.01 | 0.31 ± 0.02a | 0.22 ± 0.02 | 0.31 ± 0.01ba |
| hydroxyporoline μg ml−1 | 6.65 ± 1.02 | 9.83 ± 2.07nsd | 5.63 ± 0.93 | 8.30 ± 0.88b |
| sGAG μg ml−1 | 54.2 ± 3.87 | 49.6 ± 9.36nsd | 38.2 ± 7.96 | 42.7 ± 6.79nsd |
nsd, no significant difference to control groups.
(N = 6 for all groups, P < 0.05).
a
Significantly different from Immature AC.
b
Significantly different from serum-free cultured (ITS) immature cartilage.