Exposure to apamin and/or TRAM-34 inhibits evoked responses to SKA-31, bradykinin, and adenosine, but not SNP, in female hearts. (A) Representative tracings of coronary flow (upper) and LV developed pressure (lower) in response to acute administration of SKA-31 (0.5 and 5 µg), SNP (0.2 µg), bradykinin (BK, 1 µg), and adenosine (ADO, 10 µg) in the absence and in the continued presence of apamin (0.1 µM) and TRAM-34 (1 µM). (B–D) Histograms quantifying SKA-31, BK, ADO, and SNP evoked increases in coronary flow (B), LV systolic pressure (C), and heart rate (D) under control conditions, and then in either the presence of apamin + TRAM-34, or TRAM-34 alone. Data are presented as mean ± SEM, *P < 0.05 vs. evoked response in the absence of apamin + TRAM-34.