Table 1.

Effect of treatment with L-NNA, TRAM-34, apamin + TRAM-34, and L-NNA + apamin + TRAM-34 on basal coronary flow and heart rate

Treatment protocol	Basal flow (F2/F1)	Basal HR (R2/R1)
Control	0.91 ± 0.015	0.98 ± 0.016
TRAM-34	0.85 ± 0.017	0.95 ± 0.023
Apamin + TRAM-34	0.82 ± 0.011*	0.93 ± 0.014
L-NNA	0.68 ± 0.013*	0.91 ± 0.017*
L-NNA + Apamin+ TRAM-34	0.64 ± 0.019*	0.88 ± 0.012*

Data represent the ratios of coronary flow and heart rate measurements obtained near the start of the experiment (i.e. after acute administration of vehicle only) and following a 25–30 min treatment with L-NNA (0.1 mM), apamin (0.1 μM), and/or TRAM-34 (1 μM). Control data were obtained from time control experiments (see Supplementary material online, Figure S3), in which no drug was added during the intervening treatment period. Data represent the means ± SEM calculated from four to nine male and female hearts in total.

*P < 0.05 vs. control as determined by one-way ANOVA and a Tukey's post hoc test.