Table 1.
Gene | Primer sequences | Size (bp) | Accession number |
Rat TGF-β1 | Forward: 5'-TACAACAGCACCCGCGACCG-3' Reverse: 5'-TGCGTTGTTGCGGTCCACCA-3' | 117 | NM_021578.2 |
Col I | Forward: 5'-ACCGGGCCGATGATGCCAAC-3' Reverse: 5'- ATGTGCGGGCGGGGTTCTTG-3' | 129 | NM_053304.1 |
GAPDH | Forward: 5'-GGGCTCTCTGCTCCTCCCTGTTC-3' | 107 | NM_017008.3 |
Reverse: 5'-ACGGCCAAATCCGTTCACACC-3' | |||
Human TGF-β1 | Forward: 5'-TGTTCGCGCTCTCGGCAGTG-3' Reverse: 5'-GCCTCGATGCGCTTCCGCTT-3' | 184 | NM_000660.4 |
Col I | Forward: 5'-GAGCGGACGCTAACCCCCTC-3' Reverse: 5'-AGGGCGGTGGCCGCTAAGAG-3' | 110 | NM_000088.3 |
GAPDH | Forward: 5'-CAGCCTCCCGCTTCGCTCTC-3' | 143 | NM_002046.4 |
Reverse: 5'-ACCAGGCGCCCAATACGACC-3' |
TGF-β1: Transforming growth factor-β1; Col I: Collagen type I; GAPDH: Glyceraldehydes-3-phosphate dehydrogenase.