Figure 5.

Changes in gene expression after treatment with 10 μg/mL oridonin for 24 h. After treatment with oridonin for 24 h, the expression of caspase-3, cytochrome c, Apaf-1 and Bax was up-regulated, whereas that of Bcl-2 was down-regulated. A: Agarose gel electrophoresis of the reverse-transcript polymerase chain reaction products; B: Results of optical density analyses of Apaf-1/β-actin, Bcl-2/β-actin, Bax/β-actin, caspase-3/β-actin, and cytochrome c/β-actin before and after treatment with oridonin (paired t test, ^aP < 0.05 vs before treatment with oridonin); C: Western blotting analysis.